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wave hs staining solution i  (Transgenomic)

 
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    Transgenomic wave hs staining solution i
    The SURVEYOR Plus Mutation Kit was used for <t>SN/WAVE-HS</t> analysis. Templates with different percentages of plasmid B (indicated as plas. B) were prepared by mixing plasmid B with plasmid A. The appearance of at least 2 adjacent peaks on the chromatograms from DHPLC analysis indicated the presence of heteroduplexes (front peak) and homoduplexes (back peak) in the heat-annealed PCR products. The front heteroduplexes are indicated by the symbol of *. Two symbols of ▾ indicate the expected 139-bp and <t>190-bp</t> <t>DNA</t> fragments after SN digestion of heat-annealed PCR products. The numbers above the peaks of the DNA ladder indicate the sizes of the DNA markers. The small peaks at 3 min were caused by noise from the system, which appeared in all chromatograms in the DHPLC analysis; therefore, were disregarded.
    Wave Hs Staining Solution I, supplied by Transgenomic, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/wave hs staining solution i/product/Transgenomic
    Average 90 stars, based on 1 article reviews
    wave hs staining solution i - by Bioz Stars, 2026-05
    90/100 stars

    Images

    1) Product Images from "Interference of Co-Amplified Nuclear Mitochondrial DNA Sequences on the Determination of Human mtDNA Heteroplasmy by Using the SURVEYOR Nuclease and the WAVE HS System"

    Article Title: Interference of Co-Amplified Nuclear Mitochondrial DNA Sequences on the Determination of Human mtDNA Heteroplasmy by Using the SURVEYOR Nuclease and the WAVE HS System

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0092817

    The SURVEYOR Plus Mutation Kit was used for SN/WAVE-HS analysis. Templates with different percentages of plasmid B (indicated as plas. B) were prepared by mixing plasmid B with plasmid A. The appearance of at least 2 adjacent peaks on the chromatograms from DHPLC analysis indicated the presence of heteroduplexes (front peak) and homoduplexes (back peak) in the heat-annealed PCR products. The front heteroduplexes are indicated by the symbol of *. Two symbols of ▾ indicate the expected 139-bp and 190-bp DNA fragments after SN digestion of heat-annealed PCR products. The numbers above the peaks of the DNA ladder indicate the sizes of the DNA markers. The small peaks at 3 min were caused by noise from the system, which appeared in all chromatograms in the DHPLC analysis; therefore, were disregarded.
    Figure Legend Snippet: The SURVEYOR Plus Mutation Kit was used for SN/WAVE-HS analysis. Templates with different percentages of plasmid B (indicated as plas. B) were prepared by mixing plasmid B with plasmid A. The appearance of at least 2 adjacent peaks on the chromatograms from DHPLC analysis indicated the presence of heteroduplexes (front peak) and homoduplexes (back peak) in the heat-annealed PCR products. The front heteroduplexes are indicated by the symbol of *. Two symbols of ▾ indicate the expected 139-bp and 190-bp DNA fragments after SN digestion of heat-annealed PCR products. The numbers above the peaks of the DNA ladder indicate the sizes of the DNA markers. The small peaks at 3 min were caused by noise from the system, which appeared in all chromatograms in the DHPLC analysis; therefore, were disregarded.

    Techniques Used: Mutagenesis, Plasmid Preparation

    The SURVEYOR Plus Mutation Kit was used for SN/WAVE-HS. Templates with different percentages of the DNA from blood B (designated as bld. B) were prepared by mixing the DNA from blood B with the DNA from blood A. The front heteroduplex peaks detected by DHPLC are indicated by *. ▾ indicates the expected 39-bp and 190-bp DNA fragments after SN digestion. The numbers above the peaks of the DNA ladder indicated the sizes of the DNA markers.
    Figure Legend Snippet: The SURVEYOR Plus Mutation Kit was used for SN/WAVE-HS. Templates with different percentages of the DNA from blood B (designated as bld. B) were prepared by mixing the DNA from blood B with the DNA from blood A. The front heteroduplex peaks detected by DHPLC are indicated by *. ▾ indicates the expected 39-bp and 190-bp DNA fragments after SN digestion. The numbers above the peaks of the DNA ladder indicated the sizes of the DNA markers.

    Techniques Used: Mutagenesis

    (A) Results from SN/WAVE-HS analysis. (B) Results from DHPLC analysis. The upper and lower panels of Figure 6A and Figure 6B display the results for 143B cells and the cybrid cells, respectively. In Figure 6A, the results for the DNA samples without (uncut) and with (cut) SN digestion were also compared. ▾ indicates the peaks that appeared in the chromatograms of uncut DNA but were not observed in the chromatograms of the corresponding cut DNA for all DNA samples. ▽ indicates the peaks >100 bp only present in the chromatograms of the uncut DNA of both total DNA and mtDNA (m), but not the cut DNA. Such peaks for the mtDNA were considerably smaller than those of total DNA. ↓ indicates the peaks <100 bp that were present in the chromatograms of the uncut total DNA but absent in those of the uncut mtDNA. The patterns of peaks in the chromatograms from uncut DNA and cut DNA displayed marked differences. The chromatograms of the cut DNA samples displayed numerous small peaks. One obvious peak (indicated by *) appeared for the cut DNA of whole cells but was absent for mtDNA. In Figure 6B, the small but identifiable heteroduplex peaks detected by DHPLC are indicated by #.
    Figure Legend Snippet: (A) Results from SN/WAVE-HS analysis. (B) Results from DHPLC analysis. The upper and lower panels of Figure 6A and Figure 6B display the results for 143B cells and the cybrid cells, respectively. In Figure 6A, the results for the DNA samples without (uncut) and with (cut) SN digestion were also compared. ▾ indicates the peaks that appeared in the chromatograms of uncut DNA but were not observed in the chromatograms of the corresponding cut DNA for all DNA samples. ▽ indicates the peaks >100 bp only present in the chromatograms of the uncut DNA of both total DNA and mtDNA (m), but not the cut DNA. Such peaks for the mtDNA were considerably smaller than those of total DNA. ↓ indicates the peaks <100 bp that were present in the chromatograms of the uncut total DNA but absent in those of the uncut mtDNA. The patterns of peaks in the chromatograms from uncut DNA and cut DNA displayed marked differences. The chromatograms of the cut DNA samples displayed numerous small peaks. One obvious peak (indicated by *) appeared for the cut DNA of whole cells but was absent for mtDNA. In Figure 6B, the small but identifiable heteroduplex peaks detected by DHPLC are indicated by #.

    Techniques Used:



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    The SURVEYOR Plus Mutation Kit was used for <t>SN/WAVE-HS</t> analysis. Templates with different percentages of plasmid B (indicated as plas. B) were prepared by mixing plasmid B with plasmid A. The appearance of at least 2 adjacent peaks on the chromatograms from DHPLC analysis indicated the presence of heteroduplexes (front peak) and homoduplexes (back peak) in the heat-annealed PCR products. The front heteroduplexes are indicated by the symbol of *. Two symbols of ▾ indicate the expected 139-bp and <t>190-bp</t> <t>DNA</t> fragments after SN digestion of heat-annealed PCR products. The numbers above the peaks of the DNA ladder indicate the sizes of the DNA markers. The small peaks at 3 min were caused by noise from the system, which appeared in all chromatograms in the DHPLC analysis; therefore, were disregarded.
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    The SURVEYOR Plus Mutation Kit was used for <t>SN/WAVE-HS</t> analysis. Templates with different percentages of plasmid B (indicated as plas. B) were prepared by mixing plasmid B with plasmid A. The appearance of at least 2 adjacent peaks on the chromatograms from DHPLC analysis indicated the presence of heteroduplexes (front peak) and homoduplexes (back peak) in the heat-annealed PCR products. The front heteroduplexes are indicated by the symbol of *. Two symbols of ▾ indicate the expected 139-bp and <t>190-bp</t> <t>DNA</t> fragments after SN digestion of heat-annealed PCR products. The numbers above the peaks of the DNA ladder indicate the sizes of the DNA markers. The small peaks at 3 min were caused by noise from the system, which appeared in all chromatograms in the DHPLC analysis; therefore, were disregarded.
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    The SURVEYOR Plus Mutation Kit was used for <t>SN/WAVE-HS</t> analysis. Templates with different percentages of plasmid B (indicated as plas. B) were prepared by mixing plasmid B with plasmid A. The appearance of at least 2 adjacent peaks on the chromatograms from DHPLC analysis indicated the presence of heteroduplexes (front peak) and homoduplexes (back peak) in the heat-annealed PCR products. The front heteroduplexes are indicated by the symbol of *. Two symbols of ▾ indicate the expected 139-bp and <t>190-bp</t> <t>DNA</t> fragments after SN digestion of heat-annealed PCR products. The numbers above the peaks of the DNA ladder indicate the sizes of the DNA markers. The small peaks at 3 min were caused by noise from the system, which appeared in all chromatograms in the DHPLC analysis; therefore, were disregarded.
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    Image Search Results


    The SURVEYOR Plus Mutation Kit was used for SN/WAVE-HS analysis. Templates with different percentages of plasmid B (indicated as plas. B) were prepared by mixing plasmid B with plasmid A. The appearance of at least 2 adjacent peaks on the chromatograms from DHPLC analysis indicated the presence of heteroduplexes (front peak) and homoduplexes (back peak) in the heat-annealed PCR products. The front heteroduplexes are indicated by the symbol of *. Two symbols of ▾ indicate the expected 139-bp and 190-bp DNA fragments after SN digestion of heat-annealed PCR products. The numbers above the peaks of the DNA ladder indicate the sizes of the DNA markers. The small peaks at 3 min were caused by noise from the system, which appeared in all chromatograms in the DHPLC analysis; therefore, were disregarded.

    Journal: PLoS ONE

    Article Title: Interference of Co-Amplified Nuclear Mitochondrial DNA Sequences on the Determination of Human mtDNA Heteroplasmy by Using the SURVEYOR Nuclease and the WAVE HS System

    doi: 10.1371/journal.pone.0092817

    Figure Lengend Snippet: The SURVEYOR Plus Mutation Kit was used for SN/WAVE-HS analysis. Templates with different percentages of plasmid B (indicated as plas. B) were prepared by mixing plasmid B with plasmid A. The appearance of at least 2 adjacent peaks on the chromatograms from DHPLC analysis indicated the presence of heteroduplexes (front peak) and homoduplexes (back peak) in the heat-annealed PCR products. The front heteroduplexes are indicated by the symbol of *. Two symbols of ▾ indicate the expected 139-bp and 190-bp DNA fragments after SN digestion of heat-annealed PCR products. The numbers above the peaks of the DNA ladder indicate the sizes of the DNA markers. The small peaks at 3 min were caused by noise from the system, which appeared in all chromatograms in the DHPLC analysis; therefore, were disregarded.

    Article Snippet: DNA samples before (uncut groups) and after SN digestion (cut groups) were analyzed simultaneously by the ultraviolet (UV) detector (WAVE System) and the fluorescent detector (WAVE HS System), which was coupled with a device to mix the WAVE HS Staining Solution I (Transgenomic) with DNA samples before injection, at the column temperature of 50°C under the mode of Double-strand (DS) Multiple Fragments.

    Techniques: Mutagenesis, Plasmid Preparation

    The SURVEYOR Plus Mutation Kit was used for SN/WAVE-HS. Templates with different percentages of the DNA from blood B (designated as bld. B) were prepared by mixing the DNA from blood B with the DNA from blood A. The front heteroduplex peaks detected by DHPLC are indicated by *. ▾ indicates the expected 39-bp and 190-bp DNA fragments after SN digestion. The numbers above the peaks of the DNA ladder indicated the sizes of the DNA markers.

    Journal: PLoS ONE

    Article Title: Interference of Co-Amplified Nuclear Mitochondrial DNA Sequences on the Determination of Human mtDNA Heteroplasmy by Using the SURVEYOR Nuclease and the WAVE HS System

    doi: 10.1371/journal.pone.0092817

    Figure Lengend Snippet: The SURVEYOR Plus Mutation Kit was used for SN/WAVE-HS. Templates with different percentages of the DNA from blood B (designated as bld. B) were prepared by mixing the DNA from blood B with the DNA from blood A. The front heteroduplex peaks detected by DHPLC are indicated by *. ▾ indicates the expected 39-bp and 190-bp DNA fragments after SN digestion. The numbers above the peaks of the DNA ladder indicated the sizes of the DNA markers.

    Article Snippet: DNA samples before (uncut groups) and after SN digestion (cut groups) were analyzed simultaneously by the ultraviolet (UV) detector (WAVE System) and the fluorescent detector (WAVE HS System), which was coupled with a device to mix the WAVE HS Staining Solution I (Transgenomic) with DNA samples before injection, at the column temperature of 50°C under the mode of Double-strand (DS) Multiple Fragments.

    Techniques: Mutagenesis

    (A) Results from SN/WAVE-HS analysis. (B) Results from DHPLC analysis. The upper and lower panels of Figure 6A and Figure 6B display the results for 143B cells and the cybrid cells, respectively. In Figure 6A, the results for the DNA samples without (uncut) and with (cut) SN digestion were also compared. ▾ indicates the peaks that appeared in the chromatograms of uncut DNA but were not observed in the chromatograms of the corresponding cut DNA for all DNA samples. ▽ indicates the peaks >100 bp only present in the chromatograms of the uncut DNA of both total DNA and mtDNA (m), but not the cut DNA. Such peaks for the mtDNA were considerably smaller than those of total DNA. ↓ indicates the peaks <100 bp that were present in the chromatograms of the uncut total DNA but absent in those of the uncut mtDNA. The patterns of peaks in the chromatograms from uncut DNA and cut DNA displayed marked differences. The chromatograms of the cut DNA samples displayed numerous small peaks. One obvious peak (indicated by *) appeared for the cut DNA of whole cells but was absent for mtDNA. In Figure 6B, the small but identifiable heteroduplex peaks detected by DHPLC are indicated by #.

    Journal: PLoS ONE

    Article Title: Interference of Co-Amplified Nuclear Mitochondrial DNA Sequences on the Determination of Human mtDNA Heteroplasmy by Using the SURVEYOR Nuclease and the WAVE HS System

    doi: 10.1371/journal.pone.0092817

    Figure Lengend Snippet: (A) Results from SN/WAVE-HS analysis. (B) Results from DHPLC analysis. The upper and lower panels of Figure 6A and Figure 6B display the results for 143B cells and the cybrid cells, respectively. In Figure 6A, the results for the DNA samples without (uncut) and with (cut) SN digestion were also compared. ▾ indicates the peaks that appeared in the chromatograms of uncut DNA but were not observed in the chromatograms of the corresponding cut DNA for all DNA samples. ▽ indicates the peaks >100 bp only present in the chromatograms of the uncut DNA of both total DNA and mtDNA (m), but not the cut DNA. Such peaks for the mtDNA were considerably smaller than those of total DNA. ↓ indicates the peaks <100 bp that were present in the chromatograms of the uncut total DNA but absent in those of the uncut mtDNA. The patterns of peaks in the chromatograms from uncut DNA and cut DNA displayed marked differences. The chromatograms of the cut DNA samples displayed numerous small peaks. One obvious peak (indicated by *) appeared for the cut DNA of whole cells but was absent for mtDNA. In Figure 6B, the small but identifiable heteroduplex peaks detected by DHPLC are indicated by #.

    Article Snippet: DNA samples before (uncut groups) and after SN digestion (cut groups) were analyzed simultaneously by the ultraviolet (UV) detector (WAVE System) and the fluorescent detector (WAVE HS System), which was coupled with a device to mix the WAVE HS Staining Solution I (Transgenomic) with DNA samples before injection, at the column temperature of 50°C under the mode of Double-strand (DS) Multiple Fragments.

    Techniques: